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#1 Massimo Tosi

Massimo Tosi

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Posté 11 mars 2005 - 02:15

Torino 11 March 2005
(ITALY)

Few photos of the tissues of a mouse embryo 21 days old.
Excuse me for my English language.
I do not speak and read French, but you could answer me in Franch however.
Best Regards,

Massimo

PS.
I have other three photos but I can not to send them because I have no enough room in the attachment.
Why?
There is another way to post them?

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#2 Michel

Michel

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Posté 11 mars 2005 - 06:53

Summary
Subject: Longitudinal section of a mouse embryo 21 days old.
Prepared slide.
Staining: E.E.
Illumination: Tungsten lamp --- 6 Volt. --- Koehler mode
Filter(s): Cyan
Lomo Trinocular MFN-11 7x
Camera: Zenit 122
Photographic enlargement factor: 2.4x
Film : ILFORD HP5 PLUS B&W ISO 400/27° DXN 24x36mm. 24 EXP
Film Development:
Ilford ILFOTEC HC
Solution 1 : 31 Time: 6.30 min.
Temperature: 20°C
Fixing
Ilford HYPAM
Solution 1 : 4 Time: 5 min.
Temperature: 20°C
Washing: flowing water for 30 min.
Hints:
Without doubt the photos are not exceptional.
All of them suffer from over-exposure which compromises the range of the grey shades by flattening the
image. However they are not been processed.
The choice of the film kind it is not one of the most lucky, despite the grain seems rather limited.
The photos have been done with the “B” exposure by varying the lighting time so the light temperature is
non plain during the exposure. I hope to improve the results by changing the camera, my Canon AE-1
instead of the standard Zenit 122, so I’ll have a larger range of exposure times.
I think to remain in the B&W field because I like it and I like to develop and to print the films by myself too.
I also think that would be a happy binomial: “Microscope” & “Dark Room”.
With B&W film I could also test the effect of some coloured filters (like green filter, for instance).
Just a last hint: the specimen shows some “artefacts” as the detachment of the outer part (epidermis) of the
skin.
With my Best Regards,
Massimo
PS.
Excuse me for the English language.
But you can answer me in French. My wife can help me because she can read it.
Thank you.
GUT
Objective 10x plan-achromatic
Variable magnification factor: 1.1x
Exposure time: 0.50 sec.
Theoretic enlargement on the film plane : 26.4x

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#3 Michel

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Posté 11 mars 2005 - 06:55

GUT
Objective 10x plan-achromatic
Variable magnification factor: 2.5x
Exposure time: 0.70 sec.
Theoretic enlargement on the film plane : 60.0x

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  • Sans_titre2.JPG

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#4 Michel

Michel

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Posté 11 mars 2005 - 06:57

MUSCULAR TISSUE
Objective 40x plan-achromatic
Variable magnification factor: 1.1x
Exposure time: 1.00 sec.
Theoretic enlargement on the film plane : 105.6x

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  • Sans_titre3.JPG

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#5 Michel

Michel

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Posté 11 mars 2005 - 06:58

SKIN
Objective 4x plan-achromatic
Variable magnification factor: 2.5x
Exposure time: 0.70 sec.
Theoretic enlargement on the film plane : 24.0x

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#6 Michel

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Posté 11 mars 2005 - 07:04

Bonjour Pegaso,

Bravo pour ce travail embryologique.

Cordialement.
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